Intertek ASG Laboratory, Manchester, UK

Intertek ASG Case Study:

Biotechnological and Biological Product Analysis

Intertek ASG has experience of providing full analytical packages or individual analyses. Analyses are applicable from early through to late stage product development programmes, and typical examples have included the following analytical technology areas:

Determination of protein intact molecular weight by mass spectrometry
Peptide Mapping (fingerprinting) including N- and C-terminal sequencing by mass spectrometry
Amino acid composition including determination of the extinction coefficient
Electrophoretic and isoform patterns determined by isoelectric focusing and SDS-PAGE
Liquid chromatographic analysis using suitable chromatographic procedures
N-terminal Sequencing (using Edman Chemistry)
Sufhydryl group and disulfide bridge assignment by MS and MS/MS analysis and sites of mismatched disulfide (-S-S-) forms
Determination of carbohydrate content (neutral sugars, amino sugars, sialic acids)
Analysis of the structure of carbohydrate chains, oligosaccharide patterns and the glycosylation sites on the polypeptide chain
Identification of other post-translational modifications (PTMs, e.g. acetylation, phosphorylation, addition of lipids)
Product-related impurities including degradation products (e.g. de-amidation, acetylation, oxidation, or carbamylation)