Intertek ASG Laboratory

Case Study: LC-MS/MS

Post Translational Modification (PTM), peptide sequencing and phosphorylation site determination scans for drug discovery, drug development, and proteomics

Return to LC-MS/MS Laboratory Capabilities

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Figure 1: Post Translation Modification (PTM) Scan for Phosphopeptides

In the top pane, the blue trace represents the specific PTM scan for phosphopeptides, which shows the specificity of PTM identification when compared to a general MS scan for all peptides (shown in the top red trace).

After a polarity change at 24 minutes, the high-resolution scan enables charge state assignment and accurate mass determination.

The MS/MS spectrum of peptide 591.8 (2+ charge) shows the peptide sequence and the site of phosphorylation.

Figure 2: Peptide Sequencing and Phophorylation Site Determination